Thursday, 27 March 2014

Compound P binds with X to form the complex PX. A series of solutions was prepared with the total P concentration remaining fixed at 1.00 x10-5

) Compound P binds with X to form the complex PX. A series of solutions was prepared with 
the total P concentration remaining fixed at 1.00 x10-5
 M in all solutions. Both P and X do not absorb at 
437 nm, but PX has an absorption maximum at 437 nm. The following table shows how the absorbance 
at 437 nm in a 5.00 cm cell depends of [X], using the assumption that [X]is approximately equal to XT 
because XT >>*P+. Make a Scatchard plot of ΔA/*X+ versus ΔA in order to find the equilibrium constant K 
for this binding event. 

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